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glucose complete media  (Thermo Fisher)


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    Thermo Fisher glucose complete media
    Glucose Complete Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose complete media/product/Thermo Fisher
    Average 99 stars, based on 44581 article reviews
    glucose complete media - by Bioz Stars, 2026-03
    99/100 stars

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    NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated A549 cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.

    Journal: Materials Today Bio

    Article Title: Photocrosslinkable lung dECM hydrogels promote stiffness-dependent lung cancer growth and chemoresistance

    doi: 10.1016/j.mtbio.2026.102838

    Figure Lengend Snippet: NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated A549 cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.

    Article Snippet: 1 × 10 6 A549 cells (ATCC) were seeded at P90 in T75 culture flasks and cultured using 10 mL complete A549 media (Dulbecco's modified eagle medium (DMEM) + 10 % FCS + 1 % pen/strep) in a tissue culture incubator at 37 °C.

    Techniques: Staining, Activity Assay, Metabolic Assay, Microscopy